New insights into the evolution of wheat avenin-like proteins in wild emmer wheat (Triticum dicoccoides)

Fifteen full-length wheat grain avenin-like protein coding genes (TaALP) were identified on chromosome arms 7AS, 4AL, and 7DS of bread wheat with each containing five genes. Besides the a- and b-type ALPs, a c type was identified in the current paper. Both a and b types have two subunits, named x and y types. The five genes on each of the three chromosome arms consisted of two x-type genes, two y-type genes, and one c-type gene. The a-type genes were typically of 520 bp in length, whereas the b types were of 850 bp in length, and the c type was of 470 bp in length. The ALP gene transcript levels were significantly up-regulated in Blumeria graminis f. sp. tritici (Bgt)-infected wheat grain caryopsis at early grain filling. Wild emmer wheat [(WEW), Triticum dicoccoides] populations were focused on in our paper to identify allelic variations of ALP genes and to study the influence of natural selection on certain alleles. Consequently, 25 alleles were identified for TdALP-bx-7AS, 13 alleles were identified for TdALP-ax-7AS, 7 alleles were identified for TdALP-ay-7AS, and 4 alleles were identified for TdALP-ax-4AL. Correlation studies on TdALP gene diversity and ecological stresses suggested that environmental factors contribute to the ALP polymorphism formation in WEW. Many allelic variants of ALPs in the endosperm of WEW are not present in bread wheat and therefore could be utilized in breeding bread wheat varieties for better quality and elite plant defense characteristics

Final Report: 03-LW-005 Space-Time Secure Communications for Hostile Environments

The development of communications for highly reverberative environments is a major concern for both the private and military sectors whether the application is aimed at the securing a stock order or stalking hostile in a tunnel or cave. Other such environments can range from a hostile urban setting populated with a multitude of buildings and vehicles to the simple complexity of a large number of sound sources that are common in the stock exchange, or military operations in an environment with a topographic features hills, valleys, mountains or even a maze of buried water pipes attempting to transmit information about any chemical anomalies in the water system servicing a city or town. These inherent obstructions cause transmitted signals to reflect, refract and disperse in a multitude of directions distorting both their shape and arrival times at network receiver locations. Imagine troops attempting to communicate on missions in underground caves consisting of a maze of chambers causing multiple echoes with the platoon leader trying to issue timely commands to neutralize terrorists. This is the problem with transmitting information in a complex environment. Waves are susceptible to multiple paths and distortions created by a variety of possible obstructions, which may exist in the particular propagation medium. This is precisely the communications problem we solve using the physics of wave propagation to not only mitigate the noxious effects created by the hostile medium, but also to utilize it in a constructive manner enabling a huge benefit in communications. We employ time-reversal (T/R) communications to accomplish this task. This project is concerned with the development of secure communications techniques that can operate even in the most extreme conditions while maintaining a secure link between host and client stations. We developed an approach based on the concept of time-reversal (T/R) signal processing. In fact, the development of T/R communication systems is a recent signal processing research area dominated by applying these techniques to communicate in hostile environments. The fundamental concept is based on time-reversing the impulse response or Green's function characterizing the uncertain communications channel to mitigate deleterious dispersion and multipath effects. In this project, we have performed proof-of-principle experiments to demonstrate point-to-point and array-to-point communications by first establishing the basic theory to define and solve the underlying multi-channel communications problem and then developing various realizations of the resulting T/R receivers. We showed that not only do these receivers perform well in a hostile environment, but they also can be implemented with a ''1-bit'' analog-to-digital (A/D) converter design structure. We validated these results by performing the proof-of-principle acoustic communications simulations and experiments in air as well as electromagnetic (EM) simulation/experiments. It was shown that the resulting T/R receivers are capable of extracting the transmitted coded sequence from noisy microphone array measurements with zero (bit) error. We chose to perform the bulk of our work in the acoustics medium for simplicity in implementation and cost compared to the EM modality. However, we did perform some simple simulations and experiments using the LLNL micro-impulse transceiver system

Wheat avenin-like protein and its significant Fusarium Head Blight resistant functions

Wheat Avenin-like proteins (TaALP) are atypical storage proteins belonging to the Prolamin superfamily. Previous studies on ALPs have focused on the proteins’ positive effects on dough strength, whilst no correlation has been made between TaALPs and the plant immune system. Here, we performed genome-wide characterization of ALP encoding genes in bread wheat. In silico analyses indicated the presence of critical peptides in TaALPs that are active in the plant immune system. Pathogenesis-related nucleotide motifs were also identified in the putative promoter regions of TaALP encoding genes. RT-PCR was performed on TaALP and previously characterised pathogenesis resistance genes in developing wheat caryopses under control and Fusarium graminearum infection conditions. The results showed that TaALP and NMT genes were upregulated upon F. graminearum inoculation. mRNA insitu hybridization showed that TaALP genes were expressed in the embryo, aleurone and sub-aleurone layer cells. Seven TaALP genes were cloned for the expression of recombinant proteins in Escherichia coli, which displayed significant inhibitory function on F. graminearum under anti-fungal tests. In addition, FHB index association analyses showed that allelic variations of two ALP genes on chromosome 7A were significantly correlated with FHB symptoms. Over-expression of an ALP gene on chromosome 7A showed an enhanced resistance to FHB. Yeast two Hybridization results revealed that ALPs have potential proteases inhibiting effect on metacaspases and beta-glucosidases. A vital infection process related pathogen protein, F. graminearum Beta-glucosidase was found to interact with ALPs. Our study is the first to report a class of wheat storage protein or gluten protein with biochemical functions. Due to its abundance in the grain and the important multi-functions, the results obtained in the current study are expected to have a significant impact on wheat research and industry

Digital Signal Processing

Contains an introduction and reports on seventeen research projects.U.S. Navy - Office of Naval Research (Contract N00014-77-C-0266)Amoco Foundation FellowshipU.S. Navy - Office of Naval Research (Contract N00014-81-K-0742)National Science Foundation (Grant ECS80-07102)U.S. Army Research Office (Contract DAAG29-81-K-0073)Hughes Aircraft Company FellowshipAmerican Edwards Labs. GrantWhitaker Health Sciences FundPfeiffer Foundation GrantSchlumberger-Doll Research Center FellowshipGovernment of Pakistan ScholarshipU.S. Navy - Office of Naval Research (Contract N00014-77-C-0196)National Science Foundation (Grant ECS79-15226)Hertz Foundation Fellowshi

Digital Signal Processing Group

Contains an introduction and reports on nineteen research projects.U.S. Navy - Office of Naval Research (Contract N00014-77-C-0266)U.S. Navy - Office of Naval Research (Contract N00014-81-K-0742)National Science Foundation (Grant ECS80-07102)Bell Laboratories FellowshipAmoco Foundation FellowshipU.S. Navy - Office of Naval Research (Contract N00014-77-C-0196)Schlumberger-Doll Research Center FellowshipToshiba Company FellowshipVinton Hayes FellowshipHertz Foundation Fellowshi

Digital Signal Processing

Contains introduction and reports on seventeen research projects.U.S. Navy - Office of Naval Research (Contract N00014-81-K-0742)U.S. Navy - Office of Naval Research (Contract N00014-77-C-0266)National Science Foundation (Grant ECS80-07102)Bell Laboratories FellowshipAmoco Foundation FellowshipSchlumberger-Doll Research Center FellowshipSanders Associates, Inc.Toshiba Company FellowshipM.I.T. Vinton Hayes FellowshipHertz Foundation Fellowshi

Enhanced gas sensing and photocatalytic activity of reduced graphene oxide loaded TiO2 nanoparticles

In the present study, we have evaluated the gas sensing and photocatalytic activity of reduced graphene oxide (rGO) conjugated titanium dioxide (TiO2) nanoparticles (NPs) formed by the hydrothermal method. The as-synthesized rGO-TiO2 nanocomposite were characterized for the physicochemical properties such as the nature of crystallinity, functionalization, and morphology by making use of the powder X-ray diffraction, Fourier transform-infrared spectroscopy, and scanning electron microscopy, respectively. On testing the gas sensing properties, we found that the rGO-TiO2 nanocomposite can serve as the chemoresistive-type sensor because of its sensitivity and selectivity towards different concentrations of hydrogen and oxygen at room temperature conditions. However, the rGO-TiO2 sensor’s response and recovery speed towards hydrogen and oxygen needs further optimization. Test of photocatalytic activity of TiO2-rGO catalyst for the removal of two model contaminant dyes, RhB and MB showed effective removal, with respective degradation percentages of about 80 and 90% within the first 50 min of irradiation under visible light irradiation. Besides, MB was more effectively degraded using TiO2-rGO than pure TiO2 during the first 30 min of irradiation and this enhanced activity can be attributed to the increased capacity of light absorption, the efficiency of charge carriers separation, and the specific surface area maintained by the rGO-TiO2 nanocomposite to effectively utilize the photo-generated holes (h+) and superoxide radicals (O2−radical dot), responsible for the degradation of the dye. Based on the overall analysis, the formation of rGO-TiO2 nanocomposite can significantly improve the gas sensing and photocatalytic properties of TiO2 NPs and thus can be potential for practical applications in future nanotechnology

Non-escaping frost tolerant QTL linked genetic loci at reproductive stage in six wheat DH populations

Reproductive stage frost poses a major constraint for wheat production in countries such as Australia. However, little progress has been made in identifying key genes to overcome the constraint. In the present study, a severe frost event hit two large-scale field trials consisting of six doubled haploid (DH) wheat populations at reproductive stage (young microspore stage) in Western Australia, leading to the identification of 30 robust frost QTL on 17 chromosomes. The major 18 QTL with the phenotype variation over 9.5% were located on 13 chromosomes including 2A, 2B, 2D, 3A, 4A, 4B, 4D, 5A, 5D, 6D, 7A, 7B and 7D. Most frost QTL were closely linked to the QTL of anthesis, maturity, Zadok stages as well as linked to anthesis related genes. Out of those, six QTL were repetitively detected on the homologous regions on 2B, 4B, 4D, 5A, 5D, 7A in more than two populations. Results showed that the frost damage is associated with alleles of Vrn-A1a, Vrn-D1a, Rht-B1b, Rht-D1b, and the high copy number of Ppd-B1. However, anthesis QTL and anthesis related genes of Vrn-B1a and TaFT3-1B on chromosomes 5B and 1B did not lead to frost damage, indicating that these early-flowering phenotype related genes are compatible with frost tolerance and thus can be utilised in breeding. Our results also indicate that wild-type alleles Rht-B1a and Rht-D1a can be used when breeding for frost-tolerant varieties without delaying flowering time